Coding

Part:BBa_K571003:Design

Designed by: Lu-Chu Ke   Group: iGEM11_TzuChiU_Formosa   (2011-10-03)


acsCD/pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3147
    Illegal PstI site found at 57
    Illegal PstI site found at 243
    Illegal PstI site found at 693
    Illegal PstI site found at 2529
    Illegal PstI site found at 2859
    Illegal PstI site found at 2985
    Illegal PstI site found at 4058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3147
    Illegal PstI site found at 57
    Illegal PstI site found at 243
    Illegal PstI site found at 693
    Illegal PstI site found at 2529
    Illegal PstI site found at 2859
    Illegal PstI site found at 2985
    Illegal PstI site found at 4058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3147
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3147
    Illegal PstI site found at 57
    Illegal PstI site found at 243
    Illegal PstI site found at 693
    Illegal PstI site found at 2529
    Illegal PstI site found at 2859
    Illegal PstI site found at 2985
    Illegal PstI site found at 4058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3147
    Illegal PstI site found at 57
    Illegal PstI site found at 243
    Illegal PstI site found at 693
    Illegal PstI site found at 2529
    Illegal PstI site found at 2859
    Illegal PstI site found at 2985
    Illegal PstI site found at 4058
    Illegal NgoMIV site found at 576
    Illegal NgoMIV site found at 1652
    Illegal NgoMIV site found at 2772
    Illegal AgeI site found at 788
    Illegal AgeI site found at 2079
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

acsCD is long base pairs gene, so more than one primer is used during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results.


Source

  • organism: Gluconacetobacter hansenii ATCC 23769
  • GenBank: AB091060.1

References